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- Patents > 2008-10-30 > SECTION C CHEMISTRY; METALLURGY > BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING > MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
Method for producing steviol synthetase gene and steviol
Abstract
To identify the steviol synthetase gene for research and development of metabolic engineering, for example, for increasing a stevioside producing ability. It was successfully found that CYP714A2 derived from Arabidopsis thaliana is surprisingly steviol synthetase. Furthermore, a system in which a large amount of steviol can be biosynthesized was developed by overexpressing this steviol synthetase gene. The steviol synthetase gene is, for example, a polynucleotide encoding a protein which includes the amino acid sequence of SEQ ID NO: 2.
Filed on: 2008-03-26; Application Number: 12078016
Applicant(s)
Shinjiro YamaguchiTakahito Nomura
Hiroshi Magome
Yuji Kamiya