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Method for producing steviol synthetase gene and steviol

Abstract

To identify the steviol synthetase gene for research and development of metabolic engineering, for example, for increasing a stevioside producing ability. It was successfully found that CYP714A2 derived from Arabidopsis thaliana is surprisingly steviol synthetase. Furthermore, a system in which a large amount of steviol can be biosynthesized was developed by overexpressing this steviol synthetase gene. The steviol synthetase gene is, for example, a polynucleotide encoding a protein which includes the amino acid sequence of SEQ ID NO: 2.

Filed on: 2008-03-26; Application Number: 12078016

Applicant(s)

Shinjiro Yamaguchi
Takahito Nomura
Hiroshi Magome
Yuji Kamiya

Agent

BIRCH STEWART KOLASCH BIRCH

Assignee

Riken